Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G
نویسندگان
چکیده
منابع مشابه
Electrochemical detection of kinase-catalyzed phosphorylation using ferrocene-conjugated ATP.
Adenosine-5'-[gamma-ferrocene] triphosphate is exploited as a co-substrate for the phosphorylation of the surface-immobilized peptide C-SIYRRGSRRWRKL by protein kinase C, in which the gamma-ferrocene phosphate is transferred to the peptide and then detected by cyclic voltammetry.
متن کاملElectrochemical probing of HIV enzymes using ferrocene-conjugated peptides on surfaces.
One of the current pathways to develop inhibitors that target different steps in the life cycle of the human immunodeficiency virus (HIV) is blocking the function of the HIV-related proteins such as HIV-1 integrase (HIV-1 IN), HIV-1 reverse transcriptase (HIV-1 RT) and HIV-1 protease (HIV-1 PR), which are essential proteins that control the ability of HIV to infect cells, produce new copies of ...
متن کاملColumn enzyme immunoassay for secretory immunoglobulin A in serum.
This enzyme immunoassay for specific measurement of secretory immunoglobulin A concentrations in human serum involves use of a small chromatographic column as a solid-phase. Serum samples are incubated for 2 h with beta-D-galactosidase-labeled antibody to secretory component, then passed through a 0.1-mL Sepharose 4B column containing antibodies to human immunoglobulin A. After the column is wa...
متن کاملDEMONSTRATION OF LOCAL ANTITOXOPLASMIC IMMUNOGLOBULIN G PRODUCTION IN OCULAR TOXOPLASMOSIS, TRACED BY ENZYME IMMUNOASSAY OF AQUEOUS HUMOR AND SERUM
Forty patients with cataracts, as well as 40 patients with proven ocular toxoplasmosis were enrolled in this prospective clinical study. Serum IgG and aqueous IgG in both groups were measured by enzyme-linked immunosorbent assay (ELISA) and the corresponding ratios were calculated. Serum IgG/aqueous humor IgG ratio was less than 100 in the patient group and more than 100 in the control gro...
متن کاملCapillary electrophoresis-based immunoassay for the determination of brevetoxin-B in shellfish using electrochemical detection.
Neurotoxic shellfish poisoning is a significant food-borne disease caused by potent cyclic polyether toxins (brevetoxins) that accumulate in the flesh of shellfish. Here we report a new procedure for brevetoxin analysis by capillary electrophoresis-based immunoassay (CE-IA) with electrochemical (EC) detection. In this method, after the competitive immunoreaction in liquid phase, the horseradish...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Electrochemistry
سال: 2000
ISSN: 1344-3542,2186-2451
DOI: 10.5796/electrochemistry.68.872